If the player loses, then they can not activate it again due to the transition to the Game Over screen where the screen goes black, then the losing message, brain, and options to return to map, Zen Garden, try the level again, and watch an ad to earn power ups will appear. However, it can also happen if the player finishes or loses the level.However, the player can still re-activate it afterwards. This is because it can tell the player a zombie is about to enter the player's house.
Activated Turbo Button (v1.0.81+) Trivia